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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
14/10/2014 |
Actualizado : |
07/11/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
BRAMBILLASCA, S.; BRITOS, A.; DELUCA, C.; FRAGA, M.; CAJARVILLE, C. |
Afiliación : |
MARTIN FRAGA COTELO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay./Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay. |
Título : |
Addition of citrus pulp and apple pomace in diets for dogs: influence on fermentation kinetics, digestion, faecal characteristics and bacterial populations. |
Fecha de publicación : |
2013 |
Fuente / Imprenta : |
Archives of Animal Nutrition, v. 67, n. 6, p. 492-502, 2013. |
ISSN : |
1745-039X. |
DOI : |
10.1080/1745039X.2013.857079. |
Idioma : |
Inglés |
Notas : |
Article history: Received 26 July 2013/ Accepted 4 October 2013. |
Palabras claves : |
FERMENTACIÓN INTESTINAL; HECES; PULPA DE CITRUS; PULPA DE MANZANA. |
Thesagro : |
ALIMENTACIÓN; BACTERIAS; DIGESTIBILIDAD; DIGESTIÓN; FIBRA; FLORA INTESTINAL; PERRO. |
Asunto categoría : |
L51 Fisiología Animal - Nutrición |
Marc : |
LEADER 01038naa a2200325 a 4500 001 1051062 005 2019-11-07 008 2013 bl uuuu u00u1 u #d 022 $a1745-039X. 024 7 $a10.1080/1745039X.2013.857079.$2DOI 100 1 $aBRAMBILLASCA, S. 245 $aAddition of citrus pulp and apple pomace in diets for dogs$binfluence on fermentation kinetics, digestion, faecal characteristics and bacterial populations.$h[electronic resource] 260 $c2013 500 $aArticle history: Received 26 July 2013/ Accepted 4 October 2013. 650 $aALIMENTACIÓN 650 $aBACTERIAS 650 $aDIGESTIBILIDAD 650 $aDIGESTIÓN 650 $aFIBRA 650 $aFLORA INTESTINAL 650 $aPERRO 653 $aFERMENTACIÓN INTESTINAL 653 $aHECES 653 $aPULPA DE CITRUS 653 $aPULPA DE MANZANA 700 1 $aBRITOS, A. 700 1 $aDELUCA, C. 700 1 $aFRAGA, M. 700 1 $aCAJARVILLE, C. 773 $tArchives of Animal Nutrition$gv. 67, n. 6, p. 492-502, 2013.
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INIA La Estanzuela (LE) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
25/09/2016 |
Actualizado : |
09/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
ARRUABARRENA, A.; BENITEZ-GALEANO, M.J.; GIAMBIASI, M.; BERTALMIO, A.; COLINA, R.; HERNÁNDEZ-RODRÍGUEZ, L. |
Afiliación : |
ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA JOSÉ BENÍTEZ-GALEANO, Laboratorio de Virología Molecular, Centro Universitario Regional Noroeste (CENUR Noroeste), Universidad de la República; MARIO ALEJANDRO GIAMBIASI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA MARIA BERTALMIO CASARIEGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, Centro Universitario Regional Noroeste (CENUR Noroeste), Universidad de la República; LESTER HERNÁNDEZ-RODRÍGUEZ, Instituto de Investigaciones en Fruticultura Tropical, La Habana, Cuba. |
Título : |
Application of a simple and affordable protocol for isolating plant total nucleic acids for RNA and DNA virus detection. |
Fecha de publicación : |
2016 |
Fuente / Imprenta : |
Journal of Virological Methods, 2016, v.237, p. 14-17. |
DOI : |
10.1016/j.jviromet.2016.08.011 |
Idioma : |
Inglés |
Notas : |
Article history: Received 30 May 2016 / Received in revised form 26 July 2016 / Accepted 14 August 2016 / Available online 16 August 2016. |
Contenido : |
ABSTRACT.
Standard molecular methods for plant virus diagnosis require the purification of RNA or DNA extracts from a large number of samples, with sufficient concentration and quality for their use in PCR, RT-PCR, or qPCR analysis. Most methods are laborious and use either hazardous and/or costly chemicals.Apreviously published protocol for RNA isolation from several plant species yields high amounts of good quality RNADNA mixture in a simple, safe and inexpensive manner. In the present work, this method was tested to obtain RNA-DNA extracts from leaves of tomato, potato and three species of citrus, and was compared with two commercial kits. The results demonstrated that this protocol offers at least comparable nucleic
acid quality, quantity and purity to those provided by commercial phenol-based or spin column systems and that are suitable to be used in PCR, RT-PCR and qPCR for virus and viroid detection. Because of its easy implementation and the use of safe and inexpensive reagents, it can be easily implemented to work in plant virus and viroid detection in different plant species.
© 2016 Elsevier B.V. All rights reserved |
Palabras claves : |
DNA; PCR; PLANT VIROID; PLANT VIRUS; PURIFICATION; RNA; RT-PCR. |
Thesagro : |
CITRUS. |
Asunto categoría : |
-- |
Marc : |
LEADER 02137naa a2200301 a 4500 001 1055729 005 2019-10-09 008 2016 bl uuuu u00u1 u #d 024 7 $a10.1016/j.jviromet.2016.08.011$2DOI 100 1 $aARRUABARRENA, A. 245 $aApplication of a simple and affordable protocol for isolating plant total nucleic acids for RNA and DNA virus detection.$h[electronic resource] 260 $c2016 500 $aArticle history: Received 30 May 2016 / Received in revised form 26 July 2016 / Accepted 14 August 2016 / Available online 16 August 2016. 520 $aABSTRACT. Standard molecular methods for plant virus diagnosis require the purification of RNA or DNA extracts from a large number of samples, with sufficient concentration and quality for their use in PCR, RT-PCR, or qPCR analysis. Most methods are laborious and use either hazardous and/or costly chemicals.Apreviously published protocol for RNA isolation from several plant species yields high amounts of good quality RNADNA mixture in a simple, safe and inexpensive manner. In the present work, this method was tested to obtain RNA-DNA extracts from leaves of tomato, potato and three species of citrus, and was compared with two commercial kits. The results demonstrated that this protocol offers at least comparable nucleic acid quality, quantity and purity to those provided by commercial phenol-based or spin column systems and that are suitable to be used in PCR, RT-PCR and qPCR for virus and viroid detection. Because of its easy implementation and the use of safe and inexpensive reagents, it can be easily implemented to work in plant virus and viroid detection in different plant species. © 2016 Elsevier B.V. All rights reserved 650 $aCITRUS 653 $aDNA 653 $aPCR 653 $aPLANT VIROID 653 $aPLANT VIRUS 653 $aPURIFICATION 653 $aRNA 653 $aRT-PCR 700 1 $aBENITEZ-GALEANO, M.J. 700 1 $aGIAMBIASI, M. 700 1 $aBERTALMIO, A. 700 1 $aCOLINA, R. 700 1 $aHERNÁNDEZ-RODRÍGUEZ, L. 773 $tJournal of Virological Methods, 2016$gv.237, p. 14-17.
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